mouse fetuin a Search Results


recombinant feta protein  (MedChemExpress)
92
MedChemExpress recombinant feta protein
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Recombinant Feta Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/AHSG%2FFetuin-A%2C+Mouse/pm39277121-80-0-6
Average 92 stars, based on 1 article reviews
recombinant feta protein - by Bioz Stars, 2026-10
92/100 stars
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fetua  (Sino Biological)
92
Sino Biological fetua
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Fetua, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+Fetuin-A+%2F+AHSG+Protein/pm39633051-700-10-15
Average 92 stars, based on 1 article reviews
fetua - by Bioz Stars, 2026-10
92/100 stars
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recombinant mouse fetuin a  (R&D Systems)
90
R&D Systems recombinant mouse fetuin a
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Recombinant Mouse Fetuin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Recombinant+Mouse+Fetuin+A%2FAHSG+Protein%2C+CF/pmc09636801-128-0-6
Average 90 stars, based on 1 article reviews
recombinant mouse fetuin a - by Bioz Stars, 2026-10
90/100 stars
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antimurine feta monoclonal antibody  (R&D Systems)
93
R&D Systems antimurine feta monoclonal antibody
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Antimurine Feta Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+Fetuin+A%2FAHSG+DuoSet+ELISA/pm27965111-397-13-19
Average 93 stars, based on 1 article reviews
antimurine feta monoclonal antibody - by Bioz Stars, 2026-10
93/100 stars
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recombinant fetuin a protein  (R&D Systems)
90
R&D Systems recombinant fetuin a protein
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Recombinant Fetuin A Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Recombinant+Mouse+Fetuin+A%2FAHSG+Protein%2C+CF/pm29439057-51-5-10
Average 90 stars, based on 1 article reviews
recombinant fetuin a protein - by Bioz Stars, 2026-10
90/100 stars
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mouse fetuin a ahsg duoset elisa  (R&D Systems)
93
R&D Systems mouse fetuin a ahsg duoset elisa
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Mouse Fetuin A Ahsg Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+Fetuin+A%2FAHSG+DuoSet+ELISA/pmc12073913-213-5-9
Average 93 stars, based on 1 article reviews
mouse fetuin a ahsg duoset elisa - by Bioz Stars, 2026-10
93/100 stars
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mouse fetuin a ahsg quantikine elisa kit  (R&D Systems)
93
R&D Systems mouse fetuin a ahsg quantikine elisa kit
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Mouse Fetuin A Ahsg Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+Fetuin+A%2FAHSG+Quantikine+ELISA+Kit/pmc11936739-51-22-27
Average 93 stars, based on 1 article reviews
mouse fetuin a ahsg quantikine elisa kit - by Bioz Stars, 2026-10
93/100 stars
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mouse fetuin a  (Elabscience Biotechnology)
92
Elabscience Biotechnology mouse fetuin a
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Mouse Fetuin A, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+FETUA+(Fetuin+A)+ELISA+Kit/pmc11494534-44-0-9
Average 92 stars, based on 1 article reviews
mouse fetuin a - by Bioz Stars, 2026-10
92/100 stars
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goat anti mouse fetuin a antibody  (R&D Systems)
90
R&D Systems goat anti mouse fetuin a antibody
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Goat Anti Mouse Fetuin A Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+Fetuin+A%2FAHSG+Antibody/pmc05384772-42-18-22
Average 90 stars, based on 1 article reviews
goat anti mouse fetuin a antibody - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
mouse fetuin a/ahsg quantikine elisa kit  (Bio-Techne corporation)
93
Bio-Techne corporation mouse fetuin a/ahsg quantikine elisa kit
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Mouse Fetuin A/Ahsg Quantikine Elisa Kit, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+fetuin+a/Mouse+Fetuin+A%2FAHSG+Quantikine+ELISA+Kit/bio-techne+corporation___mfta00
Average 93 stars, based on 1 article reviews
mouse fetuin a/ahsg quantikine elisa kit - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier
elisa kits  (RayBiotech inc)
90
RayBiotech inc elisa kits
Fig. 3. <t>FetA</t> is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and <t>recombinant</t> FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.
Elisa Kits, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant mouse fetuin a ahsg protein  (Novus Biologicals)
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Fig. 3. FetA is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and recombinant FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.

Journal: Free radical biology & medicine

Article Title: Hepatocyte-derived Fetuin-A promotes alcohol-associated liver disease in mice by inhibiting autophagy-lysosome degradation of TLR and M2 macrophage polarization.

doi: 10.1016/j.freeradbiomed.2024.09.011

Figure Lengend Snippet: Fig. 3. FetA is involved in the regulation of the Toll-like receptor signaling pathway. (A, B) Proteome profiling of liver tissue from EtOH-fed WT or FetA KO mice (n = 3 per group). (A) Volcano plot indicating the proteins that were differentially expressed (red, upregulated; blue, downregulated in the FetA KO group relative to the WT group) in a proteomic analysis of liver samples from EtOH-fed WT or FetA KO mice. (B) Gene Ontology (GO) enrichment analysis of the identified differentially expressed proteins was performed. (C) Prediction of protein–protein interactions using the STRING database showing two partner proteins, AHSG and TLR4. (D) Co-immunoprecipitation of FetA with TLR4 using anti-FetA or anti-TLR4. (E) Heatmap showing the differentially expressed proteins related to inflam matory signaling (n = 3 mice per group). Red indicates high relative expression, and blue indicates low relative expression. (F) The Tlr4 mRNA level in EtOH-fed mice was analyzed using qPCR. (G) Western blot analyses of TLR4 and its downstream proteins and the NLRP3 inflammasome in liver tissue (n = 3 Western blots for each protein). Relative protein level was quantitated and normalized with Actin (below panel). β-actin was used as a loading control. (H) The membrane protein of TLR4 in liver tissues of EtOH-fed mice was analyzed by Western blot. PM, plasma membrane. (I) RAW264.7 cells were cultured with medium containing 100 mM/L ethanol for 24 h and recombinant FetA protein (0, 12.5, 25, 50, 100 μg/mL) added in the last 12h. TLR4 and its downstream associated factors were detected using western blotting. (J) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12h. The mRNA levels of Tlr4 were analyzed using qPCR (n = 3 per group). (K) RAW264.7 cells were cultured with 100 mM/L ethanol for 24 h and/or 25 μg/mL FetA added in the last 12 h. Membrane protein of TLR4 in RAW264.7 cells was analyzed using flow cytometry. Cell membrane TLR4 was further quantified by mean fluorescence intensity (MFI). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. corre-sponding control. n.s., no significance.

Article Snippet: Recombinant FetA protein was purchased from MCE (HY-P70339).

Techniques: Protein-Protein interactions, Immunoprecipitation, Expressing, Western Blot, Control, Membrane, Clinical Proteomics, Cell Culture, Recombinant, Flow Cytometry, Fluorescence